Abstract

Molecular Study of Hepcidin HAMP (-582A/G) Gene Polymorphisms and Measurement of Serum Hepcidin Level among Sudanese Patients with Anemia of Chronic Kidney Disease

Author(s): Amged Hussen Abdelrhman, Enaam Abdelrhman Abdelgadir, Khalid Mohammed Khalid

ABSTRACT
Background: Anemia of chronic disease is anemia found in a certain chronic disease state, is typically marked by the disturbance of iron
homeostasis or hypoferremia. Chronic renal failure is currently known as Chronic Kidney Disease (CKD) or Chronic Renal Insufficiency (CRI)
implies long-standing, progressive, and irreversible renal parenchyma disease resulting in diminished renal function up to 40% to 60%.
Often, Chronic Kidney Disease is diagnosed as a result of screening of people known to be at risk of kidney problems, such as those with high blood pressure or diabetes and those with a blood relative with chronic kidney disease. This disease may also be identified when it leads to one of its recognized complications such as cardiovascular disease, anemia, or pericarditis.

Methods: Sysmex KX21 used to CBC and the Cobase411 used to iron profile. Enzyme-Linked Immunoassay (ELISA) was used to determine the level of serum hepcidin.

Results: The results show the significant statistical association observed between the hepcidin level and end-stage kidney disease. When
the measured variables compared with different polymorphisms of the HAMP gene, an insignificant relation observed.

Conclusion: This study evaluates for the first time the association between Anemia of Chronic Kidney Disease (ACKD) and Hepcidin (HAMP) genes promoter polymorphisms and show that the hepcidin HAMP (-582 A/G) AA genotype and the allele A are more frequent in
patients affected by ACKD, further investigation is needed, our data support the hypothesis and hepcidin HAMP (A/G) are important in the
pathophysiology of ACKD.